Properties of the Inorganic Orthophosphate-Adenosine Triphosphate and Adenosine Diphosphate-Adenosine Triphosphate Exchange Reactions of Digitonin Particles
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چکیده
منابع مشابه
The adenosine triphosphate-adenosine diphosphate exchange reaction of oxidative phosphorylation.
Previous reports from this laboratory have demonstrated that the ATP-Pi32 1 exchange reaction (1) and the dinitrophenolstimulated ATPase activity (2) of mitochondrial subfragments (3-9) are reflections of intermediate enzymatic steps in the complex sequence of reactions (1, 3) in which phosphorylation of ADP is coupled to electron transport in the respiratory chain. This communication concerns ...
متن کاملSynthesis of adenosine triphosphate and exchange between inorganic phosphate and adenosine triphosphate in sodium and potassium ion transport adenosine triphosphatase.
Radioactive adenosine triphosphate was synthesized transiently from adenosine diphosphate and radioactive inorganic phosphate by sodium and potassium adenosine triphosphatase from guinea pig kidney. In a first step, K+-sensitive phosphoenzyme was formed from radioactive inorganic phosphate in the presence of magnesium ion and 16 mM sodium ion. In a second step the addition to the phosphoenzyme ...
متن کاملThe Eq+librium Constants of the Adenosine Triphosphate Hydrolysis and the Adenosine Triphosphate- Citrate Lyase Reactions
The observed standard free energy change (AGibs) for the hydrolysis of the terminal pyrophosphate bond of ATP has been experimentally determined under physiological conditions using an entirely new set of reactions. The observed equilibrium constant (K,,bs) for the combined reactions of acetate kinase (EC 2.7.2.1) and phosphate acetyltransferase (EC 2.3.1.8) has been determined at 38”, pH 7.0, ...
متن کاملThe equilibrium constants of the adenosine triphosphate hydrolysis and the adenosine triphosphate-citrate lyase reactions.
The observed standard free energy change (AGibs) for the hydrolysis of the terminal pyrophosphate bond of ATP has been experimentally determined under physiological conditions using an entirely new set of reactions. The observed equilibrium constant (K,,bs) for the combined reactions of acetate kinase (EC 2.7.2.1) and phosphate acetyltransferase (EC 2.3.1.8) has been determined at 38”, pH 7.0, ...
متن کاملThe adenosine triphosphate-adenosine diphosphate exchange reaction of intact rat liver mitochondria.
Studies of the adenosine triphosphate-adcnosine diphosphate exchange reaction of phosphorylating submitochondrial particles first described by Cooper and Lehninger (l), and subsequently studied in detail by Bronk and Kielley (2), and Wadkins and Lehninger (3) have provided evidence that this exchange reaction is a manifestation of the terminal step of oxidative phosphorylation in which ADP reac...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64084-0